Introduction: Vaccination during pregnancy is a simple and effective way to protect the mother and child from infections. Immunization can protect the mother from vaccine-preventable diseases and also immunization can protect the fetus and infant directly via transferred antibodies. This is why the awareness, knowledge and attitude towards vaccination during pregnancy in women is very important. Objective: The purpose of the study was to assess the awareness, knowledge and attitudes towards vaccination during pregnancy in women of reproductive age. To raise awareness about the vaccines like Tdap, flu shot (influenza) which is mostly given to women during pregnancy. Methodology: A cross sectional questionnaire-based interview was conducted among 112 pregnant women, who attended outpatient department of obstetrics and gynecology department of Saveetha medical college, Chennai. Data were collected after informed consent and ethical clearance and it was analyzed. Results: Among the 112 reproductive women involved in the study, 20% of the participants had good awareness and knowledge about vaccination during pregnancy, 42% had satisfactory awareness and knowledge and the remaining 38% had poor awareness and knowledge. According to the results the participants between the age of 30 and 35 years had high percentage (41%) of awareness about vaccination during pregnancy and it was also observed the participants with poor education and low socioeconomic status had poor awareness and knowledge about vaccination during pregnancy. Conclusion: The awareness, knowledge and uptake of the vaccine among women are low, according to the study. Hence the women should be educated about vaccination given during pregnancy in order to promote awareness and emphasize the importance of vaccination during pregnancy.