Introduction: Urinary tract infection (UTI) is one of the most common bacterial infections in developing countries. We face difficulties in choosing antibiotics during treatment of UTI due to emerging antimicrobial resistance among the predominant uropathogens. Aims: This study was undertaken to know the prevalence and antibiogram of uropathogens in our hospital and to determine the type and antibiotic resistance pattern of the urinary pathogens. Materials and Methods: The study was carried out on 103 consecutive patients suffered from UTI admitted at in-patient department of School of Tropical Medicine, Kolkata during one year. Results: In the present study we isolated 103 bacteria in suspected cases of UTI. Among the bacterial profile of UTI, Escherichia coli was the predominant isolate (36.89%) followed by Klebsiella species, Enterococcus species, Staphylococcus species, Pseudomonas aeruginosa and Citrobacter species. We found only one each case of Acinetobacter species, Serratia and Stenotrophomonas maltophilia. Conclusion:Emerging bacterial drug resistance has both clinical and financial implications for therapeutic purpose. Spectrum of bacterial drug resistance in an institution is important for epidemiological as well as clinical purposes.