Background: Bone is often the only material remaining after decaying process of the human body. Therefore identification of age, sex is aim of anatomist, forensic expert as well as anthropologist. Present study will be helpful in medico legal cases in identification of sex with atlas vertebra 150 atlas vertebrae with known sex 98 male and 52 female were obtained. Measurements of atlas vertebrae were taken by using Vernier callipers. Mean, range, standard deviation calculated. Z test was used to find out statistical significance. Results: Demarking point regarding Distance between both tips of transverse process of atlas vertebrae in male was>86.34 and in female it was <62.98. Demarking point regarding transverse diameter of vertebral canal of atlas vertebrae in male was>32.6 and in female it was <22.07 Demarking point regarding anterioposterior diameter of vertebral canal of atlas vertebrae in male was>33.12 and in female it was <25.23 Demarking point regarding area of vertebral canal of atlas vertebrae in male was > 632.5 and in female it was < 455.8 Demarking point regarding circumference of vertebral canal of atlas vertebrae in male was > 100.45 and in female it was < 80.4. Conclusions: Parameters of atlas vertebrae more than Demarking point will be definitely of male atlas vertebrae and less than Demarking point will be of female atlas vertebrae. Present study will help in identification of sex of unknown atlas vertebra.