Abstract Background: Human identification is one of the most demanding subjects and is based on scientific principles, the object of which is to identify and register individuals for both civil and criminal purposes. Aim and Objective: To check whether there are any peculiar/common lip patterns among males and females and to establish lip prints as a tool for identification. Study Design: It’s a cross sectional Prospective study. Method: It was conducted on the children who are studying in Sixth standard to Tenth standard in the Local schools at Narketpally. 170 subjects were selected irrespective of their caste, religion, dietary habits & socioeconomic status. Observation: Among males, it was found that intersected pattern was most common in compartments 1, 3 and 4, while the least common pattern in lip compartments 1, 2, was Undetermined. On evaluation of the lip prints of the females, compartments 1, 2 exhibited intersected pattern predominantly. However, in compartment 3 and 4, branched pattern was commonest. Conclusion: Lip prints are useful in personal identification and they differ from person to person even not similar in uniovular twins. Study showed that cellophane tape and lipstick can be easily used to retrieve lip prints. Lip print analysis is a process that can provides both qualitative and quantitative results.