Date rape drugs (DRDs) are particularly toxic for the reason of their quick metabolism and covert administration. The detection and elimination of date rape drugs (DRDs) is crucial for preventing drug-facilitated sexual assault (DFSA). This study compares and contrasts the most current developments in identifying DRD metabolites.The present research offers an in-depth examination of analytical advancements for the identification of DRD metabolites in urine and blood samples. With an emphasis on medications such as ketamine, flunitrazepam (Rohypnol), and gamma-hydroxybutyric acid (GHB), we looked into the metabolic pathways and detection windows of these chemicals. The urine samples may not provide the same level of immediateness as blood samples when it comes to acute forensic examination, even though they offer longer detection periods because of slower metabolite excretion. On the other hand, blood samples are more accurate just after drug administration, which makes them essential for quick forensic analysis. This review research expands knowledge of DRD detection by offering a thorough evaluation of the accuracy of different analytical approaches in distinct biological matrices. It promotes the use of a dual-sample strategy to enhance forensic investigations and aid in the prosecution of DFSA cases by utilizing the advantages of both blood and urine examinations.