ABSTRACT
Forensic biology is an application that involves a union of people to firstly suspect the crime location, artifacts and person on basis of the data provided by the victim. The provided data is analyzed by various investigations to solve criminal and civil cases. Various techniques are implemented that can be used in forensic science such as ABO blood grouping, Variable number tandem repeats, polymerase chain reaction, short tandem repeat and single cell analysis. These techniques play a significant role to completely study the samples. The entire process of the DNA analysis is divided into 4 major parts. Nanotechnology is another major field of biotechnology is currently under study for the developments of further more techniques that can be utilized in on the spot detection of sample in no time. Distinct portable devices are already developed to carry out detection and few are under study. In the forthcoming times, the process of investigation might be at rapid rate which will prevent contamination as well as mishandling of samples. The main requirement is of bioinformatics tools in laboratories in order to handle vast samples. key messages: Investigation is key step of criminal cases and DNA technology will make this step rapid and error-free. Thus, the Development of authentic, portable and user friendly molecular techniques is the necessity of the present era for fast and accurate process of justice.