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Indian Journal of Forensic Medicine and Pathology

Volume  14, Issue 4, October – December 2021, Pages 825-830
 

Original Article

Effect of formalin fixation on DNA: A Time-Based Approach

Jyoti Gullaiya1, Naresh Kumar,2 Neeharika Srivastava3

1Research Scholar, 3Assistant Professor, School of Engineering and Sciences, GD Goenka University, Haryana 122103, 2Senior Scientific Officer, Biology Division, Forensic Science Laboratory, New Delhi 110085, India

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DOI: DOI: http://dx.doi.org/10.21088/ijfmp.0974.3383.14421.7

Abstract

A B S T RA C T
Introduction:Tissue preservation is important. From it forensic scientist may extract sufficient DNA for profiling. The most commonly used preservative found in the literature is formalin. However, it causes severe side-effects on its users as well as the environment; we need to find its replacement. Method:This study examined whether formalin could preserve soft tissues (fresh) stored at 4°C for 100 days and how DNA can be extracted from it. Qualification and Quantification of the presevred samples were done. Result:The results revealed that tissues preserved in formalin failed to generate sufficient quantity of DNA for profiling whereas tissues preserved in normal saline did so. Conclusion: The study concluded that there is a need to find an alternative to formalin which can preserve the tissue samples well and enable DNA profiling.


Corresponding Author : Neeharika Srivastava