Introduction: Accidents have become the usual occurrence in today's world. Road traffic accidents, domestic accidents, industrial accidents and railway accidents contribute to large proportion of mortality, morbidity and disability. Burns of all kinds and degrees are also considered as a type of accidents. Burns cause aesthetic problems as well as acute physical problems and if not taken proper care, they can cause serious complication in the form of secondary bacterial infection, various degrees of contractures which restrict the daily activities, septicaemia, etc. People affected are mostly of poor socioeconomic status. The cost of managing these injuries is high. In developing countries, the problem of burn injuries is more severe due to the reason that the care of burn patients requires specialized staff and medical technologies that are expensive and not always readily available. Methods: A Descriptive research approach was used for the present study. The study comprised of 30 staff nurses who fulfilled inclusive criteria and working in selected hospital. Knowledge questionnaire was used for data collection. The reliability of questionnaire was done by Guttmann’s Split Half Coefficient method. In order to obtain content validity, the tool given to 10 experts who included from the field of Medical-Surgical Nursing Department. Non Probability convenience sampling technique was used, Formal Permission was obtained from concerned authority from hospital for data collection. Result: The result showed that most of the samples under the study were between the age group of 21-30 years. 50% of samples were from general wards. Result interpreted that in pre test knowledge level regarding fluid replacement in burn was 40.28 % & in post test it was 85.6%. It is evident that calculated value of ‘t’ at 0.05 level. This indicate that structured teaching programme was effective in improving the knowledge of staff nurses. Conclusion: The present study assess the knowledge level of staff nurses working in Y.C.R. Hospital, Latur regarding the “fluid replacement in burn patients” and found that he staff nurses having 29 (96.67%) had adequate knowledge,1 (3.33%) of them had moderately adequate knowledge and only 0 (0%) of staff nurses had inadequate knowledge regarding “fluid replacement in burn patients.” Keywords: Fluid Replacement; Burns; Bacterial Infection.