AbstractAn attempt has been made to develop a new thin layer chromatographic method for analysis of ‘Dothiepin, Paroxetine, Thioridazine and Trifluoperazine’ in blood using different solvent system as mobile phase. ‘These antidepressant and antipsychotic drugs’ were extracted from blood samples using liquid-liquid extraction method and analysed by Thin Layer Chromatography. 15 different solvent systems with different concentration were introduced and various TLC plates were run in these solvent systems. The Developed plates were viewed under UV light 254 nm followed by spray of chromogenic reagent i.e., Dragendorff’s Reagent which efficaciously amplified the sensitivity without upsetting simplicity of the method. The observations in each case were thoroughly analysed and reported. The developed method is a simple, rapid, low-cost, non-destructive and reproducible which can be performed in any laboratory easily.